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Introduction br Study site and the
2018-11-06
Introduction Study site and the toll like receptor of crafts Natural resource use As Japan modernized, most potters changed the natural resources they used in their work. We studied these changes through a questionnaire. The results are displayed in Tables 1 and 2. Areas recognized as the
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Introduction Mesenchymal stem cells MSCs
2018-11-06
Introduction Mesenchymal stem p38 mapk inhibitor (MSCs) are the best studied subpopulations of stem/progenitor cells in bone marrow capable of differentiating into various nonhematopoietic cells, and they are isolated by their adherence to tissue culture surfaces. They are readily isolated from pa
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The significance of loop mediated isothermal amplification
2018-11-06
The significance of loop-mediated isothermal amplification (LAMP) method in microbial detection has been drawn increasing attentions recently [8]. It was developed by Notomi et al. in 2000 [9]. It amplifies nucleic acids within tens of minutes by self-circulation of strand displacement dihydrofolate
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The molecular mechanisms that are involved
2018-11-06
The molecular mechanisms that are involved in inducing of alternative cell fates by increasing and reducing the amount of Sox2 during reprogramming remain unclear. An interesting finding was that Oct4 changes its partner and targets genes in a dose-dependent manner in human ESCs (Stefanovic et al.,
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serine protease inhibitor Previous studies have reported tha
2018-11-06
Previous studies have reported that IL-6 binds to the heterodimeric complex gp130/IL-6 receptors, followed by the activation of JAK/STAT pathway (Gu et al., 2005; Heinrich et al., 1998; Bauer, 2009). The activated Stat3 then translocates into the nucleus, regulates the expression of different transc
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We conclude that most CD intLin SCA cells
2018-11-06
We conclude that most CD45−/intLin−SCA-1+ oxidative phosphorylation in mouse BM were not as small as previously reported. Moreover, even despite broadening our search to include larger cells as candidates, we could not find a single instance of CD45−/intLin−SCA-1+ cells that (1) expressed Oct4, (2)
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br Experimental Procedures br Author Contributions br Acknow
2018-11-06
Experimental Procedures Author Contributions Acknowledgments The authors thank Drs. J. Toga and E. Yagi (Osaka University) for expression and purification of the recombinant LM511-E8 and Professor H. Ohmori (Kyoto University) for an electrophysiological study. We also thank Dr. E. Yamasaki,
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The use of a novel Pax Venus
2018-11-06
The use of a novel Pax9Venus reporter PSC line allowed the purification of PSC-derived Pax9+ AFE for molecular analysis and enabled the comparison to embryonic Pax9+ AFE. The expression of several pharyngeal endoderm transcription factors in PSC-derived Pax9+ AFE suggests that these GS-9973 may have
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br Experimental procedures br Results
2018-11-06
Experimental procedures Results Discussion Histone clipping was reported for the first time even before these proteins received their current nomenclature (Johns, 1964; Phillips and Johns, 1959). Surprisingly however, these truncation events are still greatly understudied. Yet, from a pract
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The reporter lines allowed us to
2018-11-06
The reporter lines allowed us to observe in real time that colonies with greater than 300 Cell Cycle Compound Library displayed slightly higher expression levels of the reporter at the colonies’ outermost few cell layers (Fig. 1A, F, fluorescence profiles across colonies shown in Supplementary Fig.
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Nucleofection is a method to introduce plasmid DNA into ESC
2018-11-06
Nucleofection is a method to introduce plasmid DNA into ESC with significantly greater efficiency than electroporation or virus-mediated (lentivirus, retrovirus) delivery (Lakshmipathy et al., 2004; Aslan et al., 2006; Aluigi et al., 2006). Nucleofection achieved ~60% transient expression efficiency
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Our results also appear to be in line with earlier
2018-11-06
Our results also appear to be in line with earlier observations that ABCG2 expressing cells are overall smaller than cells positive for CK3 (de Paiva et al., 2006; Kim et al., 2004). As to the effect of oxygen, it is interesting to note that both the ABCG2+ and CK3+ were in general larger in sub-amb
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5-lipoxygenase Interestingly the migration kinetics of type
2018-11-06
Interestingly, the migration kinetics of type B astrocytes was similar to that previously described for neuroblasts (Elvira et al., 2012). This suggests that both cell types are able to respond to the same “damage signals” and that type B astrocytes could help in neuroblast migration as previously s
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Bone remodeling is a highly integrated process of resorption
2018-11-06
Bone remodeling is a highly integrated process of resorption by osteoclasts and formation of bone tissue by osteoblasts, which results in precisely balanced skeletal mass with renewal of the mineralized matrix (Ashton et al., 1980). Hartig et al. reported that a 16Hz EMF enhances osteoblast activity
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br Materials and methods br Results and discussion
2018-11-06
Materials and methods Results and discussion By analyzing surface markers of order ON-01910 isolated from PD effluents, we identified a Lin−/CD34+/CD38−/CD90+ (~0.14±0.03%) subpopulation (Fig. 1A), which are known to enrich for human HSCs. PD cells also contain phenotypic hematopoietic progen
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